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multisite® gateway® cassette  (Thermo Fisher)


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    Thermo Fisher multisite® gateway® cassette
    Multisite® Gateway® Cassette, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multisite%C2%AE+gateway%C2%AE+cassette/us07582475-682-6-10
    Average 90 stars, based on 1 article reviews
    multisite® gateway® cassette - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Vectors and methods for high throughput co-expression
    Article Snippet: To create the pRIPPER-II vector, the Multisite® Gateway® Cassette from Invitrogen's pDESTTM R4-R3 vector (catalog no. 12537-023) was amplified using polymerase chain reaction (PCR) and ligated into the pCDFDuet vector, using SacI and HindIII (yielding the pDEST-CM1 vector described in Example 4) followed by the insertion of an additional T7 promoter and Lac operator.

    Amplification:

    Article Title: Vectors and methods for high throughput co-expression
    Article Snippet: To create the pRIPPER-II vector, the Multisite® Gateway® Cassette from Invitrogen's pDESTTM R4-R3 vector (catalog no. 12537-023) was amplified using polymerase chain reaction (PCR) and ligated into the pCDFDuet vector, using SacI and HindIII (yielding the pDEST-CM1 vector described in Example 4) followed by the insertion of an additional T7 promoter and Lac operator.

    Polymerase Chain Reaction:

    Article Title: Vectors and methods for high throughput co-expression
    Article Snippet: To create the pRIPPER-II vector, the Multisite® Gateway® Cassette from Invitrogen's pDESTTM R4-R3 vector (catalog no. 12537-023) was amplified using polymerase chain reaction (PCR) and ligated into the pCDFDuet vector, using SacI and HindIII (yielding the pDEST-CM1 vector described in Example 4) followed by the insertion of an additional T7 promoter and Lac operator.



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